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Msl2 mRNA is bound by free nuclear MSL complex in Drosophila melanogaster.

ABSTRACT: In Drosophila, the global increase in transcription from the male X chromosome to compensate for its monosomy is mediated by the male-specific lethal (MSL) complex consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence-dependent recognition by the MSL complex of 150-300 high affinity sites, the spread to the majority of the X-linked genes depends on local MSL-complex concentration and active transcription. We have explored whether any additional RNA species are associated with the MSL complex. No additional roX RNA species were found, but a strong association was found between a spliced and poly-adenylated msl2 RNA and the MSL complex. Based on our results, we propose a model in which a non-chromatin-associated partial or complete MSL-complex titrates newly transcribed msl2 mRNA and thus regulates the amount of available MSL complex by feedback. This represents a novel mechanism in chromatin structure regulation.

SUBMITTER: Johansson AM

PROVIDER: S-EPMC3159442 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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msl2 mRNA is bound by free nuclear MSL complex in Drosophila melanogaster.

Johansson Anna-Mia AM Allgardsson Anders A Stenberg Per P Larsson Jan J

Nucleic acids research 20110506 15

In Drosophila, the global increase in transcription from the male X chromosome to compensate for its monosomy is mediated by the male-specific lethal (MSL) complex consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence-dependent recognition by the MSL complex of 150-300 high affinity sites, the spread to the majority of the X-linked genes depends on local MSL-complex concentration and active transcription. We have explored whether any additional RNA species ...[more]

PMID: 21551218

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