Ontology highlight
ABSTRACT:
SUBMITTER: Wacker A
PROVIDER: S-EPMC3159443 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Wacker Anna A Buck Janina J Mathieu Daniel D Richter Christian C Wöhnert Jens J Schwalbe Harald H
Nucleic acids research 20110516 15
The mfl-riboswitch regulates expression of ribonucleotide reductase subunit in Mesoplasma florum by binding to 2'-deoxyguanosine and thereby promoting transcription termination. We characterized the structure of the ligand-bound aptamer domain by NMR spectroscopy and compared the mfl-aptamer to the aptamer domain of the closely related purine-sensing riboswitches. We show that the mfl-aptamer accommodates the extra 2'-deoxyribose unit of the ligand by forming a more relaxed binding pocket than t ...[more]