Ontology highlight
ABSTRACT:
SUBMITTER: Delong T
PROVIDER: S-EPMC3161333 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Delong Thomas T Baker Rocky L RL Reisdorph Nichole N Reisdorph Richard R Powell Roger L RL Armstrong Michael M Barbour Gene G Bradley Brenda B Haskins Kathryn K
Diabetes 20110706 9
<h4>Objective</h4>To investigate autoantigens in β-cells, we have used a panel of pathogenic T-cell clones that were derived from the NOD mouse. Our particular focus in this study was on the identification of the target antigen for the highly diabetogenic T-cell clone BDC-5.2.9.<h4>Research design and methods</h4>To purify β-cell antigens, we applied sequential size exclusion chromatography and reverse-phase high-performance liquid chromatography to membrane preparations of β-cell tumors. The pr ...[more]