Multiple new site-specific recombinases for use in manipulating animal genomes.
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ABSTRACT:
SUBMITTER: Nern A
PROVIDER: S-EPMC3161616 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Publications
Multiple new site-specific recombinases for use in manipulating animal genomes.
Nern Aljoscha A Pfeiffer Barret D BD Svoboda Karel K Rubin Gerald M GM
Proceedings of the National Academy of Sciences of the United States of America 20110809 34
Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four recombinases derived from yeast--KD, B2, B3, and R--are highly active and nontoxic in Drosophila and that ...[more]
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