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Mediator subunit 12 is required for neutrophil development in zebrafish.

ABSTRACT: Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.

SUBMITTER: Keightley MC

PROVIDER: S-EPMC3162013 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Mediator subunit 12 is required for neutrophil development in zebrafish.

Keightley Maria-Cristina MC Layton Judith E JE Hayman John W JW Heath Joan K JK Lieschke Graham J GJ

PloS one 20110825 8

Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr ...[more]

PMID: 21901140

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