Patterns of gene expression in Drosophila InsP3 receptor mutant larvae reveal a role for InsP3 signaling in carbohydrate and energy metabolism.

ABSTRACT:

Background

The Inositol 1,4,5-trisphosphate receptor (InsP(3)R) is an InsP(3) gated intracellular Ca(2+)-release channel. Characterization of Drosophila mutants for the InsP(3)R has demonstrated that InsP(3)-mediated Ca(2+) release is required in Drosophila larvae for growth and viability.

Methodology/principal findings

To understand the molecular basis of these growth defects a genome wide microarray analysis has been carried out with larval RNA obtained from a strong InsP(3)R mutant combination in which 1504 independent genes were differentially regulated with a log(2) of fold change of 1 or more and P<0.05. This was followed by similar transcript analyses from InsP(3)R mutants where growth defects were either suppressed by introduction of a dominant suppressor or rescued

SUBMITTER: Kumar S 

PROVIDER: S-EPMC3162032 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature