Unknown

Dataset Information

0

New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB.


ABSTRACT: A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.

SUBMITTER: Lagrange T 

PROVIDER: S-EPMC316406 | biostudies-literature | 1998 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB.

Lagrange T T   Kapanidis A N AN   Tang H H   Reinberg D D   Ebright R H RH  

Genes & development 19980101 1


A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves  ...[more]

Similar Datasets

| S-EPMC1820453 | biostudies-literature
| S-EPMC5580651 | biostudies-literature
| S-EPMC1559668 | biostudies-literature
| S-EPMC3143612 | biostudies-literature
| S-EPMC3173786 | biostudies-literature
| S-EPMC10404688 | biostudies-literature
| S-EPMC5669633 | biostudies-literature
| S-EPMC8505008 | biostudies-literature
| S-EPMC6846656 | biostudies-literature
| S-EPMC5389538 | biostudies-literature