Ontology highlight
ABSTRACT:
SUBMITTER: Davis L
PROVIDER: S-EPMC3164693 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Davis Luther L Maizels Nancy N
PloS one 20110901 9
Targeted gene correction employs a site-specific DNA lesion to promote homologous recombination that eliminates mutation in a disease gene of interest. The double-strand break typically used to initiate correction can also result in genomic instability if deleterious repair occurs rather than gene correction, possibly compromising the safety of targeted gene correction. Here we show that single-strand breaks (nicks) and double-strand breaks both promote efficient gene correction. However, breaks ...[more]