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Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay.


ABSTRACT: Despite the emergence of the pandemic H1N1 influenza A virus in 2009, seasonal H3N2 viruses continue to co-circulate in the population and may even predominate in the coming influenza season. We describe a specific minor groove binder TaqMan assay for H3N2 viruses with a detection limit of 16.5 standard DNA copies.

SUBMITTER: Wang R 

PROVIDER: S-EPMC3164769 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay.

Wang Ruixue R   Schwartzman Louis M LM   Memoli Matthew J MJ   Taubenberger Jeffery K JK  

Diagnostic microbiology and infectious disease 20110322 2


Despite the emergence of the pandemic H1N1 influenza A virus in 2009, seasonal H3N2 viruses continue to co-circulate in the population and may even predominate in the coming influenza season. We describe a specific minor groove binder TaqMan assay for H3N2 viruses with a detection limit of 16.5 standard DNA copies. ...[more]

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