Ontology highlight
ABSTRACT:
SUBMITTER: Wang S
PROVIDER: S-EPMC3164995 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Biochemistry 20110810 35
DNA sequence-dependent conformational changes induced by the minor groove binder, distamycin, have been evaluated by polyacrylamide gel electrophoresis. The distamycin binding affinity, cooperativity, and stoichiometry with three target DNA sequences that have different sizes of alternating AT sites, ATAT, ATATA, and ATATAT, have been determined by mass spectrometry and surface plasmon resonance to help explain the conformational changes. The results show that distamycin binds strongly to and be ...[more]