Potential of pmoA amplicon pyrosequencing for methanotroph diversity studies.
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ABSTRACT:
SUBMITTER: Luke C
PROVIDER: S-EPMC3165393 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Potential of pmoA amplicon pyrosequencing for methanotroph diversity studies.
Lüke Claudia C Frenzel Peter P
Applied and environmental microbiology 20110715 17
We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Major lineages were already well covered by clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the species level. ...[more]
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