Distinct microbial communities within the endosphere and rhizosphere of Populus deltoides roots across contrasting soil types.

ABSTRACT: The root-rhizosphere interface of Populus is the nexus of a variety of associations between bacteria, fungi, and the host plant and an ideal model for studying interactions between plants and microorganisms. However, such studies have generally been confined to greenhouse and plantation systems. Here we analyze microbial communities from the root endophytic and rhizospheric habitats of Populus deltoides in mature natural trees from both upland and bottomland sites in central Tennessee. Community profiling utilized 454 pyrosequencing with separate primers targeting the V4 region for bacterial 16S rRNA and the D1/D2 region for fungal 28S rRNA genes. Rhizosphere bacteria were dominated by Acidobacteria (31%) and Alphaproteobacteria (30%), whereas most endophytes were from the Gammaproteobacte

SUBMITTER: Gottel NR 

PROVIDER: S-EPMC3165402 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature