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Limitations of spoligotyping and variable-number tandem-repeat typing for molecular tracing of Mycobacterium bovis in a high-diversity setting.

ABSTRACT: This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed.

SUBMITTER: Rodriguez-Campos S

PROVIDER: S-EPMC3165628 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Limitations of spoligotyping and variable-number tandem-repeat typing for molecular tracing of Mycobacterium bovis in a high-diversity setting.

Rodriguez-Campos Sabrina S Aranaz Alicia A de Juan Lucía L Sáez-Llorente José Luis JL Romero Beatriz B Bezos Javier J Jiménez Antonio A Mateos Ana A Domínguez Lucas L

Journal of clinical microbiology 20110713 9

This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed. ...[more]

PMID: 21752973

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