Activation of the retroviral budding factor ALIX.
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ABSTRACT:
SUBMITTER: Zhai Q
PROVIDER: S-EPMC3165844 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Activation of the retroviral budding factor ALIX.
Zhai Qianting Q Landesman Michael B MB Chung Hyo-Young HY Dierkers Adam A Jeffries Cy M CM Trewhella Jill J Hill Christopher P CP Sundquist Wesley I WI
Journal of virology 20110629 17
The cellular ALIX protein functions within the ESCRT pathway to facilitate intralumenal endosomal vesicle formation, the abscission stage of cytokinesis, and enveloped virus budding. Here, we report that the C-terminal proline-rich region (PRR) of ALIX folds back against the upstream domains and auto-inhibits V domain binding to viral late domains. Mutations designed to destabilize the closed conformation of the V domain opened the V domain, increased ALIX membrane association, and enhanced viru ...[more]
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