Ontology highlight
ABSTRACT:
SUBMITTER: Clancy BE
PROVIDER: S-EPMC3167932 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Clancy Bason E BE Behnke-Parks William M WM Andreasson Johan O L JO Rosenfeld Steven S SS Block Steven M SM
Nature structural & molecular biology 20110814 9
Kinesin-1 is an ATP-driven, processive motor that transports cargo along microtubules in a tightly regulated stepping cycle. Efficient gating mechanisms ensure that the sequence of kinetic events proceeds in the proper order, generating a large number of successive reaction cycles. To study gating, we created two mutant constructs with extended neck-linkers and measured their properties using single-molecule optical trapping and ensemble fluorescence techniques. Owing to a reduction in the inter ...[more]