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Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy.


ABSTRACT: We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population.

SUBMITTER: Smith-Moritz AM 

PROVIDER: S-EPMC3168417 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy.

Smith-Moritz Andreia M AM   Chern Mawsheng M   Lao Jeemeng J   Sze-To Wing Hoi WH   Heazlewood Joshua L JL   Ronald Pamela C PC   Vega-Sánchez Miguel E ME  

Plant methods 20110818


We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population. ...[more]

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