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Structure of aldose reductase from Giardia lamblia.

ABSTRACT: Giardia lamblia is an anaerobic aerotolerant eukaryotic parasite of the intestines. It is believed to have diverged early from eukarya during evolution and is thus lacking in many of the typical eukaryotic organelles and biochemical pathways. Most conspicuously, mitochondria and the associated machinery of oxidative phosphorylation are absent; instead, energy is derived from substrate-level phosphorylation. Here, the 1.75 Å resolution crystal structure of G. lamblia aldose reductase heterologously expressed in Escherichia coli is reported. As in other oxidoreductases, G. lamblia aldose reductase adopts a TIM-barrel conformation with the NADP(+)-binding site located within the eight ?-strands of the interior.

SUBMITTER: Ferrell M

PROVIDER: S-EPMC3169411 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Structure of aldose reductase from Giardia lamblia.

Ferrell M M Abendroth J J Zhang Y Y Sankaran B B Edwards T E TE Staker B L BL Van Voorhis W C WC Stewart L J LJ Myler P J PJ

Acta crystallographica. Section F, Structural biology and crystallization communications 20110816 Pt 9

Giardia lamblia is an anaerobic aerotolerant eukaryotic parasite of the intestines. It is believed to have diverged early from eukarya during evolution and is thus lacking in many of the typical eukaryotic organelles and biochemical pathways. Most conspicuously, mitochondria and the associated machinery of oxidative phosphorylation are absent; instead, energy is derived from substrate-level phosphorylation. Here, the 1.75 Å resolution crystal structure of G. lamblia aldose reductase heterologous ...[more]

PMID: 21904059

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Structure of a cyclin-dependent kinase from Giardia lamblia.

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Crystal structure of a macrophage migration inhibitory factor from Giardia lamblia.

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Cryo-EM structure of the nucleosome core particle containing Giardia lamblia histones.

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