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Resveratrol ameliorates TNF?-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.

ABSTRACT: Overexpression of pro-inflammatory cytokines, including tumour necrosis factor alpha (TNF?), has been implicated in the pathogenesis of anaemia of inflammation. TNF? suppresses erythroid colony formation via both direct and indirect effects on haematopoietic progenitors, often involving activation of nuclear factor (NF)-?B signalling resulting in downregulation of transcription factors critical for erythropoiesis. There is a dearth of effective and safe therapies for many patients with inflammatory anaemia. Resveratrol is a flavanol found in red wine grapes that possesses potent anti-inflammatory properties, but studies of its impact on human erythropoiesis have proven contradictory. We investigated whether resveratrol ameliorates TNF?-mediated suppression of erythropoiesis in human CD34(+) haematopoietic progenitors. We found that resveratrol partially reverses the erythroid suppressive effects of TNF?, leading to significant recovery in burst forming unit-erythroid colony formation in human CD34(+) cells. CD34(+) cells pre-incubated with resveratrol for 72?h in the presence of TNF? inhibited NF-?B activation via decreased NF-?B nuclear localization without altering total NF-?B protein levels and independent of I?B degradation. Resveratrol also significantly restored the baseline expression of erythroid transcription factors NFE2 and the GATA1/GATA2 ratio in CD34(+) cells treated with TNF?. In conclusion, resveratrol may inhibit TNF?-mediated NF-?B activation and promote erythropoiesis in primary human CD34(+) cells.

SUBMITTER: Jeong JY

PROVIDER: S-EPMC3169745 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Resveratrol ameliorates TNFα-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-κB signalling.

Jeong Jee-Yeong JY Silver Matthew M Parnes Aric A Nikiforow Sarah S Berliner Nancy N Vanasse Gary J GJ

British journal of haematology 20110718 1

Overexpression of pro-inflammatory cytokines, including tumour necrosis factor alpha (TNFα), has been implicated in the pathogenesis of anaemia of inflammation. TNFα suppresses erythroid colony formation via both direct and indirect effects on haematopoietic progenitors, often involving activation of nuclear factor (NF)-κB signalling resulting in downregulation of transcription factors critical for erythropoiesis. There is a dearth of effective and safe therapies for many patients with inflammat ...[more]

PMID: 21762122

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Dataset Information

Resveratrol ameliorates TNF?-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.

Publications

Resveratrol ameliorates TNFα-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-κB signalling.

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