Identification of genetic variants using bar-coded multiplexed sequencing.
Ontology highlight
ABSTRACT:
SUBMITTER: Craig DW
PROVIDER: S-EPMC3171277 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Publications
Identification of genetic variants using bar-coded multiplexed sequencing.
Craig David W DW Pearson John V JV Szelinger Szabolcs S Sekar Aswin A Redman Margot M Corneveaux Jason J JJ Pawlowski Traci L TL Laub Trisha T Nunn Gary G Stephan Dietrich A DA Homer Nils N Huentelman Matthew J MJ
Nature methods 20080914 10
We developed a generalized framework for multiplexed resequencing of targeted human genome regions on the Illumina Genome Analyzer using degenerate indexed DNA bar codes ligated to fragmented DNA before sequencing. Using this method, we simultaneously sequenced the DNA of multiple HapMap individuals at several Encyclopedia of DNA Elements (ENCODE) regions. We then evaluated the use of Bayes factors for discovering and genotyping polymorphisms. For polymorphisms that were either previously identi ...[more]
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