Characteristics of embB mutations in multidrug-resistant Mycobacterium tuberculosis isolates in Henan, China.

ABSTRACT:

Objectives

To determine the association between embB mutations and drug resistance, and to further investigate the mechanism of embB mutations involved in the development of ethambutol and multidrug resistance in Mycobacterium tuberculosis.

Methods

One hundred and thirty-eight multidrug-resistant clinical M. tuberculosis isolates, including 86 ethambutol-resistant and 52 ethambutol-susceptible strains, were analysed to characterize mutations within the entire coding region of the embB gene. Moreover, a two-step genotyping was performed to identify the genetic lineage.

Results

In total, 27 embB mutation types were detected in 19 distinct codons. Though a strong association was observed between embB mutations and ethambutol resistance, 19.2% of embB306 mutants and 11.5%

SUBMITTER: Shi D 

PROVIDER: S-EPMC3172039 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature