Ontology highlight
ABSTRACT:
SUBMITTER: Szeto MD
PROVIDER: S-EPMC3173205 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Szeto Mindy D MD Boissel Sandrine J S SJ Baker David D Thyme Summer B SB
The Journal of biological chemistry 20110721 37
Homing endonucleases have great potential as tools for targeted gene therapy and gene correction, but identifying variants of these enzymes capable of cleaving specific DNA targets of interest is necessary before the widespread use of such technologies is possible. We identified homologues of the LAGLIDADG homing endonuclease I-AniI and their putative target insertion sites by BLAST searches followed by examination of the sequences of the flanking genomic regions. Amino acid substitutions in the ...[more]