The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
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ABSTRACT:
SUBMITTER: Wong LC
PROVIDER: S-EPMC3176278 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
Wong Li Chin LC Costa Alexandre A McLeod Ian I Sarkeshik Ali A Yates John J Kyin Saw S Perlman David D Schedl Paul P
PloS one 20110919 9
The Orb CPEB protein regulates translation of localized mRNAs in Drosophila ovaries. While there are multiple hypo- and hyperphosphorylated Orb isoforms in wild type ovaries, most are missing in orb(F303), which has an amino acid substitution in a buried region of the second RRM domain. Using a proteomics approach we identified a candidate Orb kinase, Casein Kinase 2 (CK2). In addition to being associated with Orb in vivo, we show that ck2 is required for orb functioning in gurken signaling and ...[more]
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