High-contrast Cu(I)-selective fluorescent probes based on synergistic electronic and conformational switching.

ABSTRACT: The design of fluorescent probes for the detection of redox-active transition metals such as Cu(I/II) is challenging due to potentially interfering metal-induced non-radiative deactivation pathways. By using a ligand architecture with a built-in conformational switch that maximizes the change in donor potential upon metal binding and an electronically decoupled tunable pyrazoline fluorophore as acceptor, we systematically optimized the photoinduced electron transfer (PET) switching behavior of a series of Cu(I)-selective probes and achieved an excellent fluorescence enhancement of greater than 200-fold. Crystal structure analysis combined with NMR solution studies revealed significant conformational changes of the ligand framework upon Cu(I) coordination. The photophysical data are consist

SUBMITTER: Chaudhry AF 

PROVIDER: S-EPMC3176711 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature