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Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.


ABSTRACT: Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8?nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA interactions. Further crystallographic investigation of a NusB-NusE-dsRNA complex reveals a heretofore unobserved dsRNA binding site contiguous with the BoxA binding site. We propose that the observed dsRNA represents BoxB RNA, as both single-stranded BoxA and double-stranded BoxB components are present in the classical lambda antitermination site. Combining these data with known interactions amongst antitermination factors suggests a specific model for the assembly of the complete antitermination complex.

SUBMITTER: Stagno JR 

PROVIDER: S-EPMC3177189 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.

Stagno Jason R JR   Altieri Amanda S AS   Bubunenko Mikhail M   Tarasov Sergey G SG   Li Jess J   Court Donald L DL   Byrd R Andrew RA   Ji Xinhua X  

Nucleic acids research 20110607 17


Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA i  ...[more]

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