Identification, localization, and relative quantitation of pseudouridine in RNA by tandem mass spectrometry of hydrolysis products.
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ABSTRACT: The constitutional isomers uridine (U) and pseudouridine (Ψ) cannot be distinguished from each other by simple mass measurements of RNA or its fragments because the conversion of U into Ψ is a "mass-silent" post-transcriptional modification. Here we propose a new mass spectrometry based method for identification, localization, and relative quantitation of Ψ in RNA consisting of ∼20 nucleotides that does not require chemical labeling. Our approach takes advantage of the different fragmentation behavior of uridine (N-glycosidic bond) and pseudouridine (C-glycosidic bond) residues in RNA upon collisionally activated dissociation.
SUBMITTER: Taucher M
PROVIDER: S-EPMC3180913 | biostudies-literature |
REPOSITORIES: biostudies-literature
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