Hepatocyte Growth Factor stimulated cell scattering requires ERK and Cdc42-dependent tight junction disassembly.
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ABSTRACT:
SUBMITTER: Togawa A
PROVIDER: S-EPMC3182281 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Publications
Hepatocyte Growth Factor stimulated cell scattering requires ERK and Cdc42-dependent tight junction disassembly.
Togawa Akashi A Sfakianos Jeffery J Ishibe Shuta S Suzuki Sayuri S Fujigaki Yoshihide Y Kitagawa Masatoshi M Mellman Ira I Cantley Lloyd G LG
Biochemical and biophysical research communications 20100820 2
The mechanism by which Hepatocyte Growth Factor (HGF) induces tight junction disassembly prior to cell scattering is largely unknown. Here, we show that HGF stimulates rapid loss of the TJ assembly protein Par6 from the TJ in an Erk-dependent manner. Erk activation by HGF is found to mediate the interaction of Par6 with GTP-loaded Cdc42. The Cdc42 GTPase activating protein cdGAP is shown to interact with Pkcζ at baseline and prevent Par6-Cdc42 association. Erk, by phosphorylating cdGAP at threon ...[more]
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