Dataset Information

Identification of novel ?-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.

ABSTRACT: Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated ?-synuclein (?-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determine known and novel isoforms of ?-syn in brain tissue homogenates. N-terminally acetylated full-length ?-syn (Ac-?-syn?????) and two N-terminally acetylated C-terminally truncated forms of ?-syn (Ac-?-syn????? and Ac-?-syn?????) were found. The different forms of ?-syn were further studied by Western blotting in brain tissue homogenates from the temporal cortex Brodmann area 36 (BA36) and the dorsolateral prefrontal cortex BA9 derived from controls, patients with DLB and PD with dementia (PDD). Quantification of ?-syn in each brain tissue fraction was performed using a novel enzyme-linked immunosorbent assay (ELISA).

SUBMITTER: Ohrfelt A

PROVIDER: S-EPMC3183298 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Identification of novel α-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.

Ohrfelt Annika A Zetterberg Henrik H Andersson Kerstin K Persson Rita R Secic Dzemila D Brinkmalm Gunnar G Brinkmalm Gunnar G Wallin Anders A Mulugeta Ezra E Francis Paul T PT Vanmechelen Eugeen E Aarsland Dag D Ballard Clive C Blennow Kaj K Westman-Brinkmalm Ann A

Neurochemical research 20110616 11

Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated α-synuclein (α-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determi ...[more]

PMID: 21674238

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Dataset Information

Identification of novel ?-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.

Publications

Identification of novel α-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.

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