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Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.


ABSTRACT: BACKGROUND:Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS:Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics. CONCLUSIONS:This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.

SUBMITTER: Paule S 

PROVIDER: S-EPMC3184050 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.

Paule Sarah S   Meehan Katie K   Rainczuk Adam A   Stephens Andrew N AN   Nie Guiying G  

Proteome science 20110901 1


<h4>Background</h4>Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.<h4>Results</h4>Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.<h4>Conclusions</h4>This technique enables the analysis of secreted proteins of cultured cell  ...[more]

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2017-04-18 | GSE80623 | GEO