Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.
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ABSTRACT:
SUBMITTER: Paule S
PROVIDER: S-EPMC3184050 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.
Paule Sarah S Meehan Katie K Rainczuk Adam A Stephens Andrew N AN Nie Guiying G
Proteome science 20110901 1
<h4>Background</h4>Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.<h4>Results</h4>Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.<h4>Conclusions</h4>This technique enables the analysis of secreted proteins of cultured cell ...[more]
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