Lac operator repeats generate a traceable fragile site in mammalian cells.
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ABSTRACT:
SUBMITTER: Jacome A
PROVIDER: S-EPMC3185343 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Lac operator repeats generate a traceable fragile site in mammalian cells.
Jacome Ariana A Fernandez-Capetillo Oscar O
EMBO reports 20110930 10
One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site through the cell cycle. By using this system, we show that Plk1-interacting checkpoint helicase (PICH) is already present at fragile sites during interphase, suggesting roles for th ...[more]
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