Ontology highlight
ABSTRACT:
SUBMITTER: Shen BW
PROVIDER: S-EPMC3185434 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Shen Betty W BW Xu Derrick D Chan Siu-Hong SH Zheng Yu Y Zhu Zhenyu Z Xu Shuang-yong SY Stoddard Barry L BL
Nucleic acids research 20110630 18
A type IIG restriction endonuclease, RM.BpuSI from Bacillus pumilus, has been characterized and its X-ray crystal structure determined at 2.35Å resolution. The enzyme is comprised of an array of 5-folded domains that couple the enzyme's N-terminal endonuclease domain to its C-terminal target recognition and methylation activities. The REase domain contains a PD-x(15)-ExK motif, is closely superimposable against the FokI endonuclease domain, and coordinates a single metal ion. A helical bundle do ...[more]