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D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.

ABSTRACT: This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

SUBMITTER: Dagar SS

PROVIDER: S-EPMC3187145 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.

Dagar Sumit S SS Kumar Sanjay S Mudgil Priti P Singh Rameshwar R Puniya Anil K AK

Applied and environmental microbiology 20110722 18

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation. ...[more]

PMID: 21784906

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