Ontology highlight
ABSTRACT:
SUBMITTER: Reith J
PROVIDER: S-EPMC3187400 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Reith Jan J Berking Anne A Mayer Christoph C
Journal of bacteriology 20110722 19
We report here the cloning and characterization of a cytoplasmic kinase of Clostridium acetobutylicum, named MurK (for murein sugar kinase). The enzyme has a unique specificity for both amino sugars of the bacterial cell wall, N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), which are phosphorylated at the 6-hydroxyl group. Kinetic analyses revealed Km values of 190 and 127 μM for MurNAc and GlcNAc, respectively, and a kcat value (65.0 s(-1)) that was 1.5-fold higher for the latte ...[more]