Ontology highlight
ABSTRACT:
SUBMITTER: Hung YP
PROVIDER: S-EPMC3190165 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Hung Yin Pun YP Albeck John G JG Tantama Mathew M Yellen Gary G
Cell metabolism 20111001 4
NADH is a key metabolic cofactor whose sensitive and specific detection in the cytosol of live cells has been difficult. We constructed a fluorescent biosensor of the cytosolic NADH-NAD(+) redox state by combining a circularly permuted GFP T-Sapphire with a bacterial NADH-binding protein, Rex. Although the initial construct reported [NADH] × [H(+)] / [NAD(+)], its pH sensitivity was eliminated by mutagenesis. The engineered biosensor Peredox reports cytosolic NADH:NAD(+) ratios and can be calibr ...[more]