Dataset Information

A new dispersed element in the genome of the catfish Hisonotus leucofrenatus (Teleostei: Siluriformes: Hypoptopomatinae).

ABSTRACT: Eight restriction enzymes were used in the prospection and isolation of repetitive sequences in the genome of the catfish Hisonotus leucofrenatus, a siluriform fish species that presents a large heterochromatic block in the W chromosome. In the tested enzymes, only the BamHI enzyme revealed a distinct band of 224 bp G+C value of 37%. In comparative analyses with sequences already available in the GenBank, the BamHI fragment sequence showed similarity with part of a Lepidoptera transposon. Fluorescent in situ hybridization (FISH) revealed that this sequence presents a dispersed pattern in the genome of H. leucofrenatus, forming clusters in some chromosome pairs in the pericentromeric region, which frequently are rich in constitutive heterochromatin. Based on the analysis performed, it can be inferred that the HLBam fragment constitutes a genomically dispersed transposon type element. It can be considered that the findings in this study can contribute to a better understanding of the organization and distribution of transposable elements in the genome of teleost fish.

SUBMITTER: Ferreira DC

PROVIDER: S-EPMC3190325 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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A new dispersed element in the genome of the catfish Hisonotus leucofrenatus (Teleostei: Siluriformes: Hypoptopomatinae).

Ferreira Daniela Cristina DC Oliveira Claudio C Foresti Fausto F

Mobile genetic elements 20110701 2

Eight restriction enzymes were used in the prospection and isolation of repetitive sequences in the genome of the catfish Hisonotus leucofrenatus, a siluriform fish species that presents a large heterochromatic block in the W chromosome. In the tested enzymes, only the BamHI enzyme revealed a distinct band of 224 bp G+C value of 37%. In comparative analyses with sequences already available in the GenBank, the BamHI fragment sequence showed similarity with part of a Lepidoptera transposon. Fluore ...[more]

PMID: 22016856

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Project description:A very significant part of the world's freshwater ichthyofauna is represented by ancient, exceptionally diverse and cosmopolitan ray-finned teleosts of the order Siluriformes. Over the years, catfish have been established as an exemplary model for probing historical biogeography at various scales. Yet, several tantalizing gaps still exist in their phylogenetic history, timeline and mode of diversification. Here, we re-examine the phylogeny of catfish by assembling and analyzing almost all publicly available mitogenome data. We constructed an ingroup matrix of 62 full-length mitogenome sequences from 20 catfish families together with four cypriniform outgroups, spanning 15,557 positions in total. Partitioned maximum likelihood analyses and Bayesian relaxed clock dating using fossil age constraints provide some useful and novel insights into the evolutionary history of this group. Loricarioidei are recovered as the first siluriform group to diversify, rendering Neotropics the cradle of the order. The next deepest clade is the South American Diplomystoidei placed as a sister group to all the remaining Siluroidei. The two multifamilial clades of "Big Asia" and "Big Africa" are also recovered, albeit nodal support for the latter is poor. Within "Big Asia", Bagridae are clearly polyphyletic. Other interfamilial relationships, including Clariidae + Heteropneustidae, Doradidae + Auchenipteridae and Ictaluridae + Cranoglanididae are robustly resolved. Our chronogram shows that siluriforms have a Pangaean origin, at least as far back as the Early Cretaceous. The inferred timeline of the basal splits corroborates the "Out-of-South America" hypothesis and accords well with the fossil record. The divergence of Siluroidei most likely postdated the final separation of Africa and South America. An appealing case of phylogenetic affinity elaborated by biogeographic dispersal is exemplified by the Early Paleogene split between the Southeast Asian Cranoglanididae and Ictaluridae, with the latter radiating into North America's freshwater realm by Eocene. The end of Cretaceous probably concludes the major bout of diversification at the family level while with the dawn of the Cenozoic a prolific radiation is evident at the generic level.

Dataset Information

A new dispersed element in the genome of the catfish Hisonotus leucofrenatus (Teleostei: Siluriformes: Hypoptopomatinae).

Publications

A new dispersed element in the genome of the catfish Hisonotus leucofrenatus (Teleostei: Siluriformes: Hypoptopomatinae).

Similar Datasets

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