Ontology highlight
ABSTRACT:
SUBMITTER: Renthal R
PROVIDER: S-EPMC3192009 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Biophysical chemistry 20110827 2-3
To probe structural changes that occur when a membrane protein is transferred from lipid bilayers to SDS micelles, a fragment of bacteriorhodopsin containing transmembrane helical segments A and B was studied by fluorescence spectroscopy, molecular dynamics (MD) simulation, and stopped flow kinetics. In lipid bilayers, Förster resonance energy transfer (FRET) was observed between tyrosine 57 on helix B and tryptophans 10 and 12 on helix A. FRET efficiency decreased substantially when the peptide ...[more]