Unknown

Dataset Information

0

A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody.


ABSTRACT: Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. The aptamer consisted of two motifs (CCTTA and TGTCTWCC) separated by 2-3 bases, and the elimination of one or the other motif abrogated binding. The DNA aptamer and FLAG peptide competed for binding to the antigen-binding pocket of the M2 antibody. In addition, the aptamer eluted FLAG-tagged proteins from the antibody, suggesting a commercial application in protein purification. These findings demonstrate the feasibility of using SELEX to develop ssDNA aptamers that block the function of a specific antibody, a capability that could lead to the development of novel therapeutic modalities for patients with systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases.

SUBMITTER: Lakamp AS 

PROVIDER: S-EPMC3195435 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

altmetric image

Publications

A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody.

Lakamp Amanda S AS   Ouellette Michel M MM  

Journal of nucleic acids 20111016


Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. The aptamer consisted of two motifs (CCTTA and TGTCTWCC) separated by 2-3 bases, and the elimination of one or the other motif abrogated binding. The DNA aptamer and FLAG peptide competed for binding to the antigen-binding pocket of the M2 antibody. In addition, the aptamer eluted FLAG-tagged proteins from the antibody, sugges  ...[more]

Similar Datasets

2021-01-11 | PXD023419 | Pride
2017-06-07 | GSE99712 | GEO
| S-EPMC4897735 | biostudies-other
| S-EPMC7357423 | biostudies-literature
2022-12-12 | GSE193903 | GEO
| S-EPMC2242885 | biostudies-literature
| S-EPMC9475424 | biostudies-literature
| S-EPMC5731996 | biostudies-literature
| S-EPMC6320952 | biostudies-literature
| S-EPMC9598387 | biostudies-literature