Time-resolved NMR: extracting the topology of complex enzyme networks.
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ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC3195686 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Publications
Time-resolved NMR: extracting the topology of complex enzyme networks.
Jiang Yingnan Y McKinnon Tyler T Varatharajan Janani J Glushka John J Prestegard James H JH Sornborger Andrew T AT Schüttler Heinz-Bernd HB Bar-Peled Maor M
Biophysical journal 20101001 7
The use of nondestructive NMR spectroscopy for enzymatic studies offers unique opportunities to identify nearly all enzymatic byproducts and detect unstable short-lived products or intermediates at the molecular level; however, numerous challenges must be overcome before it can become a widely used tool. The biosynthesis of acetyl-coenzyme A (acetyl-CoA) by acetyl-CoA synthetase is used here as a case study for the development of an analytical NMR-based time-course assay platform. We describe an ...[more]
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