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A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum.

ABSTRACT: Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.

SUBMITTER: Son H

PROVIDER: S-EPMC3197617 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum.

Son Hokyoung H Seo Young-Su YS Min Kyunghun K Park Ae Ran AR Lee Jungkwan J Jin Jian-Ming JM Lin Yang Y Cao Peijian P Hong Sae-Yeon SY Kim Eun-Kyung EK Lee Seung-Ho SH Cho Aram A Lee Seunghoon S Kim Myung-Gu MG Kim Yongsoo Y Kim Jung-Eun JE Kim Jin-Cheol JC Choi Gyung Ja GJ Yun Sung-Hwan SH Lim Jae Yun JY Kim Minkyun M Lee Yong-Hwan YH Choi Yang-Do YD Lee Yin-Won YW

PLoS pathogens 20111020 10

Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits importa ...[more]

PMID: 22028654

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Project description:The Fusarium graminearum virus 1 (FgV1) causes noticeable phenotypic changes such as reduced mycelial growth, increase pigmentation, and reduced pathogenicity in its host fungi, Fusarium graminearum. Previous study showed that the numerous F. graminearum genes including regulatory factors were differentially expressed upon FgV1 infection, however, we have limited knowledge on the effect(s) of specific transcription factor (TF) during FgV1 infection in host fungus. Using gene-deletion mutant library of 657 putative TFs in F. graminearum, we transferred FgV1 by hyphal anastomosis to screen transcription factors that might be associated with viral replication or symptom induction. FgV1-infected TF deletion mutants were divided into three groups according to the mycelial growth phenotype compare to the FgV1-infected wild-type strain (WT-VI). The FgV1-infected TF deletion mutants in Group 1 exhibited slow or weak mycelial growth compare to that of WT-VI on complete medium at 5 dpi. In contrast, Group 3 consists of virus-infected TF deletion mutants showing faster mycelial growth and mild symptom compared to that of WT-VI. The hyphal growth of FgV1-infected TF deletion mutants in Group 2 was not significantly different from that of WT-VI. We speculated that differences of mycelial growth among the FgV1-infected TF deletion mutant groups might be related with the level of FgV1 RNA accumulations in infected host fungi. By conducting real-time quantitative reverse transcription polymerase chain reaction, we observed close association between FgV1 RNA accumulation and phenotypic differences of FgV1-infected TF deletion mutants in each group, i.e., increased and decreased dsRNA accumulation in Group 1 and Group 3, respectively. Taken together, our analysis provides an opportunity to identify host's regulator(s) of FgV1-triggered signaling and antiviral responses and helps to understand complex regulatory networks between FgV1 and F. graminearum interaction.

Dataset Information

A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum.

Publications

A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum.

Similar Datasets

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