Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC3201885 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Liu Yun Y Woods Nicholas T NT Kim Dewey D Sweet Michael M Monteiro Alvaro N A AN Karchin Rachel R
Nucleic acids research 20110723 19
Yeast two-hybrid (Y2H) screenings result in identification of many out-of-frame (OOF) clones that code for short (2-100 amino acids) peptides with no sequence homology to known proteins. We hypothesize that these peptides can reveal common short linear motifs (SLiMs) responsible for their selection. We present a new protocol to address this issue, using an existing SLIM detector (TEIRESIAS) as a base method, and applying filters derived from a mathematical model of SLiM selection in OOF clones. ...[more]