Unknown

Dataset Information

0

A novel metagenomic short-chain dehydrogenase/reductase attenuates Pseudomonas aeruginosa biofilm formation and virulence on Caenorhabditis elegans.


ABSTRACT: In Pseudomonas aeruginosa, the expression of a number of virulence factors, as well as biofilm formation, are controlled by quorum sensing (QS). N-Acylhomoserine lactones (AHLs) are an important class of signaling molecules involved in bacterial QS and in many pathogenic bacteria infection and host colonization are AHL-dependent. The AHL signaling molecules are subject to inactivation mainly by hydrolases (Enzyme Commission class number EC 3) (i.e. N-acyl-homoserine lactonases and N-acyl-homoserine-lactone acylases). Only little is known on quorum quenching mechanisms of oxidoreductases (EC 1). Here we report on the identification and structural characterization of the first NADP-dependent short-chain dehydrogenase/reductase (SDR) involved in inactivation of N-(3-oxo-dodecanoyl)-L-homoserine lactone (3-oxo-C(12)-HSL) and derived from a metagenome library. The corresponding gene was isolated from a soil metagenome and designated bpiB09. Heterologous expression and crystallographic studies established BpiB09 as an NADP-dependent reductase. Although AHLs are probably not the native substrate of this metagenome-derived enzyme, its expression in P. aeruginosa PAO1 resulted in significantly reduced pyocyanin production, decreased motility, poor biofilm formation and absent paralysis of Caenorhabditis elegans. Furthermore, a genome-wide transcriptome study suggested that the level of lasI and rhlI transcription together with 36 well known QS regulated genes was significantly (?10-fold) affected in P. aeruginosa strains expressing the bpiB09 gene in pBBR1MCS-5. Thus AHL oxidoreductases could be considered as potent tools for the development of quorum quenching strategies.

SUBMITTER: Bijtenhoorn P 

PROVIDER: S-EPMC3202535 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

altmetric image

Publications

A novel metagenomic short-chain dehydrogenase/reductase attenuates Pseudomonas aeruginosa biofilm formation and virulence on Caenorhabditis elegans.

Bijtenhoorn Patrick P   Mayerhofer Hubert H   Müller-Dieckmann Jochen J   Utpatel Christian C   Schipper Christina C   Hornung Claudia C   Szesny Matthias M   Grond Stephanie S   Thürmer Andrea A   Brzuszkiewicz Elzbieta E   Daniel Rolf R   Dierking Katja K   Schulenburg Hinrich H   Streit Wolfgang R WR  

PloS one 20111026 10


In Pseudomonas aeruginosa, the expression of a number of virulence factors, as well as biofilm formation, are controlled by quorum sensing (QS). N-Acylhomoserine lactones (AHLs) are an important class of signaling molecules involved in bacterial QS and in many pathogenic bacteria infection and host colonization are AHL-dependent. The AHL signaling molecules are subject to inactivation mainly by hydrolases (Enzyme Commission class number EC 3) (i.e. N-acyl-homoserine lactonases and N-acyl-homoser  ...[more]

Similar Datasets

| S-EPMC26797 | biostudies-literature
| S-EPMC6902481 | biostudies-literature
| S-EPMC3406104 | biostudies-literature
| S-EPMC24797 | biostudies-literature
| S-EPMC5511546 | biostudies-literature
| S-EPMC3536714 | biostudies-literature
| S-EPMC8223063 | biostudies-literature
| S-EPMC5700365 | biostudies-literature
| S-EPMC2669342 | biostudies-literature
| S-EPMC7707567 | biostudies-literature