Novel adiponectin variants identified in type 2 diabetic patients reveal multimerization and secretion defects.

ABSTRACT: ADIPOQ, encoding adiponectin, is a candidate gene for type 2 diabetes (T2D) identified by genome-wide linkage analyses with supporting evidence showing the protein function in sensitizing insulin actions. In an endeavor to characterize candidate genes causing T2D in Thai patients, we identified 10 novel ADIPOQ variations, several of which were non-synonymous variations observed only in the patients. To examine the impact of these non-synonymous variations on adiponectin structure and biochemical characteristics, we conducted a structural analysis of the wild-type and variant proteins by in silico modeling and further characterized biochemical properties of the variants with predicted structural abnormalities from the modeling by molecular and biochemical studies. The recombinant plasmids c

SUBMITTER: Jungtrakoon P 

PROVIDER: S-EPMC3202584 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature