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Functional analysis of a putative Dothistromin toxin MFS transporter gene.

ABSTRACT: Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, dotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing dotC, or with a dotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of dotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.

SUBMITTER: Bradshaw RE

PROVIDER: S-EPMC3202781 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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Functional analysis of a putative Dothistromin toxin MFS transporter gene.

Bradshaw Rosie E RE Feng Zhilun Z Schwelm Arne A Yang Yongzhi Y Zhang Shuguang S

Toxins 20091208 2

Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, dotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing dotC, or with a dotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflu ...[more]

PMID: 22069539

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