Implementing photodissociation in an Orbitrap mass spectrometer.
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ABSTRACT:
SUBMITTER: Vasicek LA
PROVIDER: S-EPMC3202985 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Implementing photodissociation in an Orbitrap mass spectrometer.
Vasicek Lisa A LA Ledvina Aaron R AR Shaw Jared J Griep-Raming Jens J Westphall Michael S MS Coon Joshua J JJ Brodbelt Jennifer S JS
Journal of the American Society for Mass Spectrometry 20110409 6
We modified a dual pressure linear ion trap Orbitrap to permit infrared multiphoton dissociation (IRMPD) in the higher energy collisional dissociation (HCD) cell for high resolution analysis. A number of parameters, including the pressures of the C-trap and HCD cell, the radio frequency (rf) amplitude applied to the C-trap, and the HCD DC offset, were evaluated to optimize IRMPD efficiency and maintain a high signal-to-noise ratio. IRMPD was utilized for characterization of phosphopeptides, supe ...[more]
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