Ontology highlight
ABSTRACT:
SUBMITTER: Yi H
PROVIDER: S-EPMC3203590 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Yi Hana H Cho Yong-Joon YJ Won Sungho S Lee Jong-Eun JE Jin Yu Hyung H Kim Sujin S Schroth Gary P GP Luo Shujun S Chun Jongsik J
Nucleic acids research 20110831 20
Next-generation sequencing has great potential for application in bacterial transcriptomics. However, unlike eukaryotes, bacteria have no clear mechanism to select mRNAs over rRNAs; therefore, rRNA removal is a critical step in sequencing-based transcriptomics. Duplex-specific nuclease (DSN) is an enzyme that, at high temperatures, degrades duplex DNA in preference to single-stranded DNA. DSN treatment has been successfully used to normalize the relative transcript abundance in mRNA-enriched cDN ...[more]