Ontology highlight
ABSTRACT:
SUBMITTER: Liu W
PROVIDER: S-EPMC3203607 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Liu Weizhi W Jankowska-Anyszka Marzena M Piecyk Karolina K Dickson Laura L Wallace Adam A Niedzwiecka Anna A Stepinski Janusz J Stolarski Ryszard R Darzynkiewicz Edward E Kieft Jeffrey J Zhao Rui R Jones David N M DN Davis Richard E RE
Nucleic acids research 20110929 20
Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and a common downstream SL RNA sequence. The mechanism for eIF4E binding an m²²⁷G-cap is unknown. Here, we describe the first structure of an eIF4E with an m(2,2,7)G-cap and compare it to the cognate m⁷G-eIF4E complex. These structures and Nuclear Magnetic Resonance (NMR) data indicate that the nematode Ascaris suum eIF4E binds the two different caps in a similar manner except for the loss of a single hydrogen bond ...[more]