Ontology highlight
ABSTRACT:
SUBMITTER: Heller RC
PROVIDER: S-EPMC3204357 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Heller Ryan C RC Kang Sukhyun S Lam Wendy M WM Chen Shuyan S Chan Clara S CS Bell Stephen P SP
Cell 20110701 1
Proper eukaryotic DNA replication requires temporal separation of helicase loading from helicase activation and replisome assembly. Using an in vitro assay for eukaryotic origin-dependent replication initiation, we investigated the control of these events. After helicase loading, we found that the Dbf4-dependent Cdc7 kinase (DDK) but not S phase cyclin-dependent kinase (S-CDK) is required for the initial origin recruitment of Sld3 and the Cdc45 helicase-activating protein. Likewise, in vivo, DDK ...[more]