MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics.
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ABSTRACT:
SUBMITTER: Ting L
PROVIDER: S-EPMC3205343 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Publications
MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics.
Ting Lily L Rad Ramin R Gygi Steven P SP Haas Wilhelm W
Nature methods 20111002 11
Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) a ...[more]
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