Quantitative proteomics: TGFβ₂ signaling in trabecular meshwork cells.

ABSTRACT:

Purpose

Transforming growth factor beta 2 (TGFβ₂) is often elevated in the aqueous humor (AH) and trabecular meshwork (TM) of patients with primary open-angle glaucoma (POAG) and appears to contribute to POAG pathogenesis. To better understand TGFβ₂ signaling in the eye, TGFβ₂-induced proteomic changes were identified in cells cultured from the TM, a tissue involved in intraocular pressure (IOP) elevation in glaucoma.

Methods

Primary cultures of human TM cells from four donors were treated with or without TGFβ₂ (5 ng/mL) for 48 hours; then cellular protein was analyzed by liquid chromatography-mass spectrometry iTRAQ (isobaric tags for relative and absolute quantitation) technology.

Results

A total of 853 proteins were quantified. TGFβ₂ treatment significantly altered

SUBMITTER: Bollinger KE 

PROVIDER: S-EPMC3208113 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature