Amplification methods bias metagenomic libraries of uncultured single-stranded and double-stranded DNA viruses.
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ABSTRACT:
SUBMITTER: Kim KH
PROVIDER: S-EPMC3209148 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
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Amplification methods bias metagenomic libraries of uncultured single-stranded and double-stranded DNA viruses.
Kim Kyoung-Ho KH Bae Jin-Woo JW
Applied and environmental microbiology 20110916 21
Investigation of viruses in the environment often requires the amplification of viral DNA before sequencing of viral metagenomes. In this study, two of the most widely used amplification methods, the linker amplified shotgun library (LASL) and multiple displacement amplification (MDA) methods, were applied to a sample from the seawater surface. Viral DNA was extracted from viruses concentrated by tangential flow filtration and amplified by these two methods. 454 pyrosequencing was used to read t ...[more]
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