Efficiency of neutralizing antibodies targeting the CD4-binding site: influence of conformational masking by the V2 loop in R5-tropic clade C simian-human immunodeficiency virus.
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ABSTRACT:
SUBMITTER: Watkins JD
PROVIDER: S-EPMC3209380 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
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Efficiency of neutralizing antibodies targeting the CD4-binding site: influence of conformational masking by the V2 loop in R5-tropic clade C simian-human immunodeficiency virus.
Watkins Jennifer D JD Diaz-Rodriguez Juan J Siddappa Nagadenahalli B NB Corti Davide D Ruprecht Ruth M RM
Journal of virology 20110928 23
In R5-tropic clade C simian-human immunodeficiency viruses (SHIV-Cs), we identified a 3-asparagine (3N) deletion mutation in the V2 loop stem of gp120 as the major determinant of neutralization escape of the anti-CD4-binding site (anti-CD4-bs) neutralizing monoclonal antibody (nMAb) b12. However, the more potent anti-CD4-bs nMAbs VRC01 and VRC03 were not sensitive to this mutation. Using isogenic tier 1 or tier 2 proviruses differing only in the 3N mutation, we showed that this mutation might re ...[more]
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